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We support FIU researchers and students with our expertise in mass spectrometry methodology and instrumentation. Our research facilities are also accessible to other academic, government and industry users through contract-based collaborations or service-for-fee.

Instruments

We maintain state-of-the-art instrumentation enabling a broad range of analytical approaches. Our services range from small molecule synthetic identification to biological extract characterization to tissue imaging. Complex mixtures can be investigated using traditional chromatographic separations prior to mass analysis or they can be analyzed using the new high resolution FT-ICR instrument to obtain exact mass identifications.

  • Bruker Solarix 7.0T

    Ionization Sources

    • Matrix Assisted Laser Desorption Ionization (MALDI)
    • Electrospray Ionization (ESI)


    Mass Analyzer

    • Fourier Transform Ion Cyclotron Resonance (FT-ICR)
      • Resolution: > 1,000,000 m/Δm
      • Accuracy: < 1 ppm
    • Collision Induced Dissociation (CID) capable


    Applications

    • Top-Down/Bottom-Up Proteomics
    • MALDI Imaging
    • Petroleomics
    • Natural Product Analysis
    • Top-down Protein Sequencing
    • Metabolite Profiling
    • Post-translational Modification (PTM) Analysis
    • Drug Distribution


    Bruker Solarix 7.0T website

  • ionTOF TOF.SIMS 5

    Primary Ion Sources

    • Bismuth Liquid Metal Ion Source (Bi-LMIS)
    • Argon Cluster Ion Source


    Mass Analyzer: Reflectron ToF

    • Resolution: >10,000 m/Δm
    • Spatial Resolution: <300 nm


    Applications

    • High Resolution Chemical Imaging
    • Depth Profiling
    • Small Molecule Characterization


    ionTOF TOF.SIMS 5 website

  • Bruker Autoflex III

    Ionization Sources

    • Matrix Assisted Laser Desorption Ionization (MALDI)


    Mass Analyzer Modes

    • Linear Time of Flight (ToF)
      • Resolution: >5,000 m/Δm
      • Accuracy: <20 ppm
    • Reflectron Time of Flight
      • Resolution: >20,000 m/Δm
      • Accuracy: <5 ppm
    • ToF/ToF (CID or LIFT fragmentation)


    Sensitivity: 500 amol

    Maximum Sampling Rate: 200 Hz


    Applications

    • Expression Proteomics
    • MALDI Imaging
    • Biotherapeutic Analysis
    • Biomarker Discovery
    • Polymer Analysis
    • Microbial Identification


    Bruker website

  • Bruker EvoQ LC-TQ

    Services

    • Ultra trace quantitative analysis by LC-MS/MS
    • Online SPE capable


    Bruker EvoQ LC-TQ website

  • Agilent 5973 GC-MS

    Services: Identification of volatile and semivolatile compounds via database search

  • Bruker EvoQ GC-TQ

    Services: Ultra trace quantitative analysis by GC-MS/MS

    Bruker EvoQ GC-TQ website

Services

We provide fee-for-service and contract-based collaborative research. For nonacademic users, this is an opportunity to have access to high-end mass spectrometry services without the need to invest in instruments. Collaborations in academic research usually involve the development of new mass spectrometry methods or techniques, leading to new breakthroughs. All inquiries regarding contract-based collaborations should be addressed to facility staff.

To submit a sample, fill out a Submission Form with as much information as you can and the Proteomics Addendum if appropriate. A principal investigator signature and account number are required for FIU users. For external users, a quote will be provided upon request.

Attach your sample to the submission form and leave the sample with the submission form in the inbox in CP 174 (or in the refrigerator/freezer for temperature-sensitive samples). If you are sending samples from outside the university, please contact us regarding proper procedures and handling.

Samples will be run in the order they are received, with priority given to samples from FIU researchers. Samples for each type of analysis will be run on the days specified by the schedule posted in CP 174. Samples must be received prior to the scheduled analysis time. For 9 a.m.-12 p.m. service times, the sample should be received before 9 a.m. For 1-4 p.m. service times, the sample should be received before 12 p.m. Samples submitted after these deadlines may not be run until the next scheduled analysis time.

Review our Sample Preparation Guidelines for more information.